1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide Hydrochloride (EDC HCl): Overview and Manufacturing
1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide Hydrochloride (EDC HCl), commonly known as EDC or EDAC, is a widely used carbodiimide reagent in organic synthesis and biochemical applications. It plays a crucial role in peptide coupling reactions, specifically for forming amide bonds between carboxyl and amine groups. This reagent is preferred due to its water solubility and minimal side reactions.
Chemical Structure and Properties
Chemical Name: 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide Hydrochloride
Molecular Formula: C₈H₁₇N₃ · HCl
Molecular Weight: 191.70 g/mol
CAS Number: 25952-53-8
Physical Properties:
Property Description
Appearance White to off-white crystalline solid
Melting Point 110-115°C (Decomposes)
Solubility Soluble in water and organic solvents like DMF and DMSO
Stability Stable under dry conditions; sensitive to moisture
Applications of EDC HCl
Peptide Synthesis:
EDC HCl is extensively used in peptide coupling reactions to form amide bonds between the carboxyl groups of amino acids and the amino groups of another amino acid, leading to peptide chains.
Nucleic Acid Cross-Linking:
In molecular biology, EDC is used for cross-linking oligonucleotides, proteins, and nucleic acids, particularly in constructing DNA, RNA, and protein conjugates.
Protein Conjugation:
EDC HCl is effective in conjugating proteins to carboxyl-containing surfaces or molecules, aiding in various applications like creating bioconjugates for antibody labeling.
Activation of Carboxyl Groups:
In organic chemistry, EDC is used to activate carboxyl groups to form esters, anhydrides, or amides, making it valuable for a variety of chemical transformations.
Mechanism of Action
EDC HCl works by activating carboxyl groups, turning them into an active ester intermediate. This intermediate can then react with amines to form an amide bond. One advantage of using EDC over other coupling reagents is that the by-product, N-ethyl-N'-(3-dimethylaminopropyl)urea, is water-soluble and can be easily removed by aqueous washes.
EDC HCl Manufacturers
EDC HCl is produced by several manufacturers and suppliers worldwide. In India and Italy, there are numerous manufacturers of high-quality EDC HCl used in pharmaceuticals, biotech, and chemical industries. Here are some prominent suppliers:
Manufacturers in India:
Avantor Performance Materials India Ltd.
One of the key manufacturers of EDC HCl for laboratory and industrial use. They provide high-purity reagents for pharmaceutical and research applications.
TCI Chemicals (India) Pvt. Ltd.
TCI Chemicals specializes in fine chemicals and provides high-quality EDC HCl for laboratory research, particularly for use in peptide synthesis and bioconjugation.
Sisco Research Laboratories Pvt. Ltd. (SRL)
SRL is a leading supplier of laboratory chemicals in India and produces EDC HCl for applications in biochemistry and organic synthesis.
Merck Life Science Pvt. Ltd. (India)
A global leader in chemicals and reagents, Merck offers EDC HCl as part of its extensive product portfolio for various industries, including pharmaceuticals.
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